Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
University of California, Berkeley · QB3 · +2 more institutions
Abstract
Bacterial adaptive immunity and genome engineering involving the CRISPR (clustered regularly interspaced short palindromic repeats)-associated (Cas) protein Cas9 begin with RNA-guided DNA unwinding to form an RNA-DNA hybrid and a displaced DNA strand inside the protein. The role of this R-loop structure in positioning each DNA strand for cleavage by the two Cas9 nuclease domains is unknown. We determine molecular structures of the catalytically active Streptococcus pyogenes Cas9 R-loop that show the displaced DNA strand located near the RuvC nuclease domain active site. These protein-DNA interactions, in turn, position the HNH nuclease domain adjacent to the target DNA strand cleavage site in a conformation…
Citation impact
- FWCI
- 42.47
- Percentile
- 100%
- References
- 46
Authors
8- FJFuguo JiangCorresponding
University of California, Berkeley
- DWDavid W. TaylorCorresponding
QB3, University of California, Berkeley
- JSJanice S. Chen
University of California, Berkeley
- JEJack E. Kornfeld
Howard Hughes Medical Institute, University of California, Berkeley
- KZKaihong Zhou
Howard Hughes Medical Institute, University of California, Berkeley
Topics & keywords
- CRISPR
- Cas9
- DNA
- RNA
- Biology
- Guide RNA
- Molecular biology
- Computational biology
- Life in Land