Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes

Extracellular vesicles (EVs) have become the focus of rising interest because of their numerous functions in physiology and pathology. Cells release heterogeneous vesicles of different sizes and intracellular origins, including small EVs formed inside endosomal compartments (i.e., exosomes) and EVs of various sizes budding from the plasma membrane. Specific markers for the analysis and isolation of different EV populations are missing, imposing important limitations to understanding EV functions. Here, EVs from human dendritic cells were first separated by their sedimentation speed, and then either by their behavior upon upward floatation into iodixanol gradients or by immuno-isolation. Extensive quantitative…

• AP
  Association pour la Recherche sur le Cancer

  Award: SL220120605293
• AN
  Agence Nationale de la Recherche

  Awards: ANR-10-IDEX-0001-02 PSL*, ANR-11-LABX-0043
• FP
  Fondation pour la Recherche Médicale

  Award: DGE 20121125630