Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting

The functional maturation and preservation of hepatic cells derived from human induced pluripotent stem cells (hiPSCs) are essential to personalized in vitro drug screening and disease study. Major liver functions are tightly linked to the 3D assembly of hepatocytes, with the supporting cell types from both endodermal and mesodermal origins in a hexagonal lobule unit. Although there are many reports on functional 2D cell differentiation, few studies have demonstrated the in vitro maturation of hiPSC-derived hepatic progenitor cells (hiPSC-HPCs) in a 3D environment that depicts the physiologically relevant cell combination and microarchitecture. The application of rapid, digital 3D bioprinting to tissue…

• NI
  National Institutes of Health

  Award: NS047101
• UO
  University of California, San Diego

  Award: NS047101
• NI
  National Institute of Neurological Disorders and Stroke

  Award: P30NS047101
• NI
  National Institute of Biomedical Imaging and Bioengineering

  Awards: R21EB017876, R01EB012597