articleCurrent Protocols in BioinformaticsDec 1, 2015BRONZE OA

Protein Structure and Function Prediction Using I‐TASSER

Nankai University · University of Michigan

PubMed
Indexed incrossrefpubmed

Abstract

I-TASSER is a hierarchical protocol for automated protein structure prediction and structure-based function annotation. Starting from the amino acid sequence of target proteins, I-TASSER first generates full-length atomic structural models from multiple threading alignments and iterative structural assembly simulations followed by atomic-level structure refinement. The biological functions of the protein, including ligand-binding sites, enzyme commission number, and gene ontology terms, are then inferred from known protein function databases based on sequence and structure profile comparisons. I-TASSER is freely available as both an on-line server and a stand-alone package. This unit describes how to use the…

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Topics & keywords

Keywords
  • Threading (protein sequence)
  • Protein structure prediction
  • Computer science
  • Protein structure
  • Protein function prediction
  • Computational biology
  • Protein Data Bank (RCSB PDB)
  • Function (biology)
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