Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs
Shanghai Jiao Tong University · Fudan University · +5 more institutions
Abstract
Circular RNAs (circRNAs) represent a class of widespread and diverse endogenous RNAs that may regulate gene expression in eukaryotes. However, the regulation and function of human circRNAs remain largely unknown. Here we generate ribosomal-depleted RNA sequencing data from six normal tissues and seven cancers, and detect at least 27,000 circRNA candidates. Many of these circRNAs are differently expressed between the normal and cancerous tissues. We further characterize one abundant circRNA derived from Exon2 of the HIPK3 gene, termed circHIPK3. The silencing of circHIPK3 but not HIPK3 mRNA significantly inhibits human cell growth. Via a luciferase screening assay, circHIPK3 is observed to sponge to 9 miRNAs…
Citation impact
- FWCI
- 121.16
- Percentile
- 100%
- References
- 41
Authors
14- QZQiupeng ZhengCorresponding
Shanghai Jiao Tong University, Fudan University, Renji Hospital, Fudan University Shanghai Cancer Center, Shanghai Cancer Institute, State Key Laboratory of Oncogene and Related Genes
- CBChunyang Bao
Fudan University, Fudan University Shanghai Cancer Center
- WGWeijie Guo
Shanghai Jiao Tong University, Fudan University, Renji Hospital, Fudan University Shanghai Cancer Center, Shanghai Cancer Institute, State Key Laboratory of Oncogene and Related Genes
- SLShuyi Li
Shanghai Jiao Tong University, Fudan University, Renji Hospital, Fudan University Shanghai Cancer Center, Shanghai Cancer Institute, State Key Laboratory of Oncogene and Related Genes
- JCJie Chen
Fudan University, Fudan University Shanghai Cancer Center
Topics & keywords
- microRNA
- Circular RNA
- Biology
- Gene silencing
- RNA
- Messenger RNA
- Gene expression
- Gene