Cigarette smoking and the oral microbiome in a large study of American adults

Oral microbiome dysbiosis is associated with oral disease and potentially with systemic diseases; however, the determinants of these microbial imbalances are largely unknown. In a study of 1204 US adults, we assessed the relationship of cigarette smoking with the oral microbiome. 16S rRNA gene sequencing was performed on DNA from oral wash samples, sequences were clustered into operational taxonomic units (OTUs) using QIIME and metagenomic content was inferred using PICRUSt. Overall oral microbiome composition differed between current and non-current (former and never) smokers (P

• AC
  American Cancer Society
• UD
  U.S. Department of Veterans Affairs
• NI
  National Institutes of Health

  Awards: R01CA159036, U01CA182370, P30CA016087
• NC
  National Cancer Institute

  Awards: U01CA182370, R01CA159036, P30CA016087, R01CA164964, R21CA183887, R03CA159414