Y-box protein 1 is required to sort microRNAs into exosomes in cells and in a cell-free reaction
University of California, Berkeley · University of Oregon · +1 more institution
Abstract
Exosomes are small vesicles that are secreted from metazoan cells and may convey selected membrane proteins and small RNAs to target cells for the control of cell migration, development and metastasis. To study the mechanisms of RNA packaging into exosomes, we devised a purification scheme based on the membrane marker CD63 to isolate a single exosome species secreted from HEK293T cells. Using immunoisolated CD63-containing exosomes we identified a set of miRNAs that are highly enriched with respect to their cellular levels. To explore the biochemical requirements for exosome biogenesis and RNA packaging, we devised a cell-free reaction that recapitulates the species-selective enclosure of miR-223 in isolated…
Citation impact
- FWCI
- 29.42
- Percentile
- 100%
- References
- 69
Authors
5- MJMatthew J. Shurtleff
University of California, Berkeley
- MMMorayma M. Temoche-Diaz
University of California, Berkeley
- KKKate Karfilis
University of Oregon
- SRSayaka Ri
Howard Hughes Medical Institute, University of California, Berkeley
- RSRandy SchekmanCorresponding
Howard Hughes Medical Institute, University of California, Berkeley
Topics & keywords
- Microvesicles
- Cell biology
- Exosome
- HEK 293 cells
- RNA
- Biology
- Secretion
- Biogenesis