Phase separation of signaling molecules promotes T cell receptor signal transduction
Marine Biological Laboratory · Howard Hughes Medical Institute · +3 more institutions
Abstract
Activation of various cell surface receptors triggers the reorganization of downstream signaling molecules into micrometer- or submicrometer-sized clusters. However, the functional consequences of such clustering have been unclear. We biochemically reconstituted a 12-component signaling pathway on model membranes, beginning with T cell receptor (TCR) activation and ending with actin assembly. When TCR phosphorylation was triggered, downstream signaling proteins spontaneously separated into liquid-like clusters that promoted signaling outputs both in vitro and in human Jurkat T cells. Reconstituted clusters were enriched in kinases but excluded phosphatases and enhanced actin filament assembly by recruiting and…
Citation impact
- FWCI
- 47.70
- Percentile
- 100%
- References
- 31
Authors
10- XSXiaolei SuCorresponding
Marine Biological Laboratory, Howard Hughes Medical Institute
- JAJonathon A. DitlevCorresponding
Marine Biological Laboratory, Howard Hughes Medical Institute, Southwestern Medical Center, The University of Texas Southwestern Medical Center
- EHEnfu Hui
Marine Biological Laboratory, Howard Hughes Medical Institute
- WXWenmin Xing
Marine Biological Laboratory, Howard Hughes Medical Institute, Southwestern Medical Center, The University of Texas Southwestern Medical Center
- SBSudeep Banjade
Marine Biological Laboratory, Howard Hughes Medical Institute, Southwestern Medical Center, The University of Texas Southwestern Medical Center
Topics & keywords
- Cell biology
- Jurkat cells
- Signal transduction
- T-cell receptor
- Phosphorylation
- Actin
- Cell signaling
- Receptor