Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

We continue to uncover a wealth of information connecting microbes in important ways to human and environmental ecology. As our scientific knowledge and technical abilities improve, the tools used for microbiome surveys can be modified to improve the accuracy of our techniques, ensuring that we can continue to identify groundbreaking connections between microbes and the ecosystems they populate, from ice caps to the human body. It is important to confirm that modifications to these tools do not cause new, detrimental biases that would inhibit the field rather than continue to move it forward. We therefore demonstrated that two recently modified primer pairs that target taxonomically discriminatory regions of…

• NS
  National Science Foundation

  Awards: OCE-1233612, 1136818, 1233612, DE-AC02-06CH11357, 76RL01830
• HH
  Howard Hughes Medical Institute
• UD
  U.S. Department of Energy

  Awards: 76RL01830, AC02-06CH11357, DE-AC06-76RLO1830, DE-AC02, 06CH11357, DE-AC06-76RL01830, DE-AC02-06CH11357, DE-AC02-
• AP
  Alfred P. Sloan Foundation
• GA
  Gordon and Betty Moore Foundation

  Award: GMBF3779
• B
  Battelle

  Awards: DE-AC02-06CH11357, DE-AC06-76RL01830
• LD
  Laboratory Directed Research and Development

  Awards: 76RL01830, DE-AC02-06CH11357
• PN
  Pacific Northwest National Laboratory

  Awards: DE-AC02-06CH11357, 76RL01830, DE-AC06-76RL01830