Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol
Engelhardt Institute of Molecular Biology · Moscow Institute of Physics and Technology · +2 more institutions
Abstract
Exosomes, small (40-100 nm) extracellular membranous vesicles, attract enormous research interest because they are carriers of disease markers and a prospective delivery system for therapeutic agents. Differential centrifugation, the prevalent method of exosome isolation, frequently produces dissimilar and improper results because of the faulty practice of using a common centrifugation protocol with different rotors. Moreover, as recommended by suppliers, adjusting the centrifugation duration according to rotor K-factors does not work for "fixed-angle" rotors. For both types of rotors--"swinging bucket" and "fixed-angle"--we express the theoretically expected proportion of pelleted vesicles of a given size and…
Citation impact
- FWCI
- 10.69
- Percentile
- 100%
- References
- 39
Authors
8- MAM. A. LivshitsCorresponding
Engelhardt Institute of Molecular Biology, Moscow Institute of Physics and Technology
- EKElena Khomyakova
- EGEvgeniy G. Evtushenko
Lomonosov Moscow State University
- ВНВ. Н. Лазарев
Moscow Institute of Physics and Technology
- NANikolay A. Kulemin
Moscow Institute of Physics and Technology
Topics & keywords
- Centrifugation
- Microvesicles
- Nanoparticle tracking analysis
- Differential centrifugation
- Vesicle
- Exosome
- Sedimentation
- Extracellular vesicles