Progress in Top-Down Proteomics and the Analysis of Proteoforms
Indexed incrossrefpubmed
Abstract
From a molecular perspective, enactors of function in biology are intact proteins that can be variably modified at the genetic, transcriptional, or post-translational level. Over the past 30 years, mass spectrometry (MS) has become a powerful method for the analysis of proteomes. Prevailing bottom-up proteomics operates at the level of the peptide, leading to issues with protein inference, connectivity, and incomplete sequence/modification information. Top-down proteomics (TDP), alternatively, applies MS at the proteoform level to analyze intact proteins with diverse sources of intramolecular complexity preserved during analysis. Fortunately, advances in prefractionation workflows, MS instrumentation, and…
Citation impact
568
total citations
- FWCI
- 44.88
- Percentile
- 100%
- References
- 118
Citations per year
Authors
3Topics & keywords
Topics
Keywords
- Proteomics
- Computational biology
- Proteome
- Top-down proteomics
- Computer science
- Posttranslational modification
- Protein function
- Data science
No related works found for this paper.
Funding
- NSNational Science Foundation
- UOUniversity of Wisconsin-Madison
- NUNorthwestern University
- UOUniversity of Pittsburgh
- SNSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- NINational Institutes of HealthAwards: T32GM105538, P41GM108569
- NINational Institute of General Medical SciencesAwards: P41GM108569, T32GM105538, R01GM067193