Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

By reaching near-atomic resolution for a wide range of specimens, single-particle cryo-EM structure determination is transforming structural biology. However, the necessary calculations come at large computational costs, which has introduced a bottleneck that is currently limiting throughput and the development of new methods. Here, we present an implementation of the RELION image processing software that uses graphics processors (GPUs) to address the most computationally intensive steps of its cryo-EM structure determination workflow. Both image classification and high-resolution refinement have been accelerated more than an order-of-magnitude, and template-based particle selection has been accelerated well…

• V
  Vetenskapsrådet

  Award: 2013-5901
• MR
  Medical Research Council

  Award: MC UP A025 1013
• H2
  Horizon 2020

  Award: EINFRA-2015-1-675728