Isobaric Labeling-Based Relative Quantification in Shotgun Proteomics

Mass spectrometry plays a key role in relative quantitative comparisons of proteins in order to understand their functional role in biological systems upon perturbation. In this review, we review studies that examine different aspects of isobaric labeling-based relative quantification for shotgun proteomic analysis. In particular, we focus on different types of isobaric reagents and their reaction chemistry (e.g., amine-, carbonyl-, and sulfhydryl-reactive). Various factors, such as ratio compression, reporter ion dynamic range, and others, cause an underestimation of changes in relative abundance of proteins across samples, undermining the ability of the isobaric labeling approach to be truly quantitative.…

• NH
  National Heart, Lung, and Blood Institute

  Award: R01HL079442-09
• NI
  National Institute of General Medical Sciences

  Award: P41GM103533