A sentinel goblet cell guards the colonic crypt by triggering Nlrp6-dependent Muc2 secretion

Innate immune signaling pathways contribute to the protection of host tissue when bacterially challenged. Colonic goblet cells are responsible for generating the two mucus layers that physically separate the luminal microbiota from the host epithelium. Analysis of colonic tissues from multiple mouse strains allowed us to identify a "sentinel" goblet cell (senGC) localized to the colonic crypt entrance. This cell nonspecifically endocytoses and reacts to the TLR2/1, TLR4, and TLR5 ligands by activating the Nlrp6 inflammasome downstream of TLR- and MyD88-dependent Nox/Duox reactive oxygen species synthesis. This triggers calcium ion-dependent compound exocytosis of Muc2 mucin from the senGC and generates an…

• TS
  Torsten Söderbergs Stiftelse
• SC
  Swedish Cancer Foundation
• SF
  Stiftelsen för Strategisk Forskning
• KO
  Knut och Alice Wallenbergs Stiftelse
• V
  Vetenskapsrådet
• SU
  Sahlgrenska Universitetssjukhuset
• NI
  National Institute of Allergy and Infectious Diseases

  Award: U01AI095473