articleBloodJun 30, 2016BRONZE OA

A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation

SNSonia NestorowaFHFiona HameyBPBlanca Pijuan-SalaEDEvangelia DiamantiMSMairi Shepherd

Wellcome/MRC Cambridge Stem Cell Institute · University of Cambridge · +1 more institution

PubMed
Indexed incrossrefdatacitepubmed

Abstract

Maintenance of the blood system requires balanced cell fate decisions by hematopoietic stem and progenitor cells (HSPCs). Because cell fate choices are executed at the individual cell level, new single-cell profiling technologies offer exciting possibilities for mapping the dynamic molecular changes underlying HSPC differentiation. Here, we have used single-cell RNA sequencing to profile more than 1600 single HSPCs, and deep sequencing has enabled detection of an average of 6558 protein-coding genes per cell. Index sorting, in combination with broad sorting gates, allowed us to retrospectively assign cells to 12 commonly sorted HSPC phenotypes while also capturing intermediate cells typically excluded by…

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Topics & keywords

Topics
Keywords
  • Biology
  • Progenitor cell
  • Stem cell
  • Haematopoiesis
  • Cell biology
  • Cell sorting
  • Cellular differentiation
  • Gene expression profiling
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