Activation of STING requires palmitoylation at the Golgi

Stimulator of interferon genes (STING) is essential for the type I interferon response against DNA pathogens. In response to the presence of DNA and/or cyclic dinucleotides, STING translocates from the endoplasmic reticulum to perinuclear compartments. However, the role of this subcellular translocation remains poorly defined. Here we show that palmitoylation of STING at the Golgi is essential for activation of STING. Treatment with palmitoylation inhibitor 2-bromopalmitate (2-BP) suppresses palmitoylation of STING and abolishes the type I interferon response. Mutation of two membrane-proximal Cys residues (Cys88/91) suppresses palmitoylation, and this STING mutant cannot induce STING-dependent host defense…

• JA
  Japan Agency for Medical Research and Development

  Award: CREST
• MO
  Ministry of Education, Culture, Sports, Science and Technology

  Award: CREST
• UO
  University of Tokyo
• CR
  Core Research for Evolutional Science and Technology
• NI
  National Institute for Physiological Sciences
• LB
  Lawrence Berkeley National Laboratory