Suite2p: beyond 10,000 neurons with standard two-photon microscopy
Energy Institute · University College London · +3 more institutions
Abstract
Abstract Two-photon microscopy of calcium-dependent sensors has enabled unprecedented recordings from vast populations of neurons. While the sensors and microscopes have matured over several generations of development, computational methods to process the resulting movies remain inefficient and can give results that are hard to interpret. Here we introduce Suite2p: a fast, accurate and complete pipeline that registers raw movies, detects active cells, extracts their calcium traces and infers their spike times. Suite2p runs on standard workstations, operates faster than real time, and recovers ~2 times more cells than the previous state-of-the-art method. Its low computational load allows routine detection of…
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- References
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Authors
8- MPMarius PachitariuCorresponding
Energy Institute, University College London
- CSCarsen Stringer
Gatsby Charitable Foundation, Oxford Centre for Computational Neuroscience
- MDMario Dipoppa
Energy Institute, University College London
- SSSylvia Schröder
University College London
- LFL. Federico Rossi
University College London
Topics & keywords
- Microscope
- Microscopy
- Workstation
- Pipeline (software)
- Computer science
- Calcium imaging
- Two-photon excitation microscopy
- Process (computing)