Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain
University of California San Diego · Scripps Research Institute · +1 more institution
Abstract
The human brain has enormously complex cellular diversity and connectivities fundamental to our neural functions, yet difficulties in interrogating individual neurons has impeded understanding of the underlying transcriptional landscape. We developed a scalable approach to sequence and quantify RNA molecules in isolated neuronal nuclei from a postmortem brain, generating 3227 sets of single-neuron data from six distinct regions of the cerebral cortex. Using an iterative clustering and classification approach, we identified 16 neuronal subtypes that were further annotated on the basis of known markers and cortical cytoarchitecture. These data demonstrate a robust and scalable method for identifying and…
Citation impact
- FWCI
- 60.58
- Percentile
- 100%
- References
- 37
Authors
21- BBBlue B. LakeCorresponding
University of California San Diego
- RARizi AiCorresponding
University of California San Diego
- GEGwendolyn E. KaeserCorresponding
Scripps Research Institute, University of California San Diego
- NSNeeraj SalathiaCorresponding
Illumina (United States)
- YCYun C. Yung
Scripps Research Institute
Topics & keywords
- Human brain
- Biology
- Nucleus
- Gene
- Gene expression
- Neuroscience
- Concordance
- RNA