Visualization and analysis of gene expression in tissue sections by spatial transcriptomics

Analysis of the pattern of proteins or messengerRNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics. This typically involves the visualization of a few proteins or expressed genes at a time. We have devised a strategy, which we call "spatial transcriptomics," that allows visualization and quantitative analysis of the transcriptome with spatial resolution in individual tissue sections. By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, we demonstrate high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. Spatial…

• ÅW
  Åke Wiberg Stiftelse
• RS
  Ragnar Söderbergs stiftelse
• TS
  Torsten Söderbergs Stiftelse
• JS
  Jeanssons Stiftelser
• SF
  Stiftelsen för Strategisk Forskning
• C
  Cancerfonden
• KI
  Karolinska Institutet
• KO
  Knut och Alice Wallenbergs Stiftelse
• V
  Vetenskapsrådet
• SF
  Science for Life Laboratory
• UM
  Uppsala Multidisciplinary Center for Advanced Computational Science