Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein–protein interaction module

Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded…

• NR
  National Research Foundation
• KH
  Korea Health Industry Development Institute

  Award: HI14C0042
• KB
  Korea Basic Science Institute
• NR
  National Research Foundation of Korea

  Award: HI14C0042