mito -QC illuminates mitophagy and mitochondrial architecture in vivo

Autophagic turnover of mitochondria, termed mitophagy, is proposed to be an essential quality-control (QC) mechanism of pathophysiological relevance in mammals. However, if and how mitophagy proceeds within specific cellular subtypes in vivo remains unclear, largely because of a lack of tractable tools and models. To address this, we have developed "mito-QC," a transgenic mouse with a pH-sensitive fluorescent mitochondrial signal. This allows the assessment of mitophagy and mitochondrial architecture in vivo. Using confocal microscopy, we demonstrate that mito-QC is compatible with classical and contemporary techniques in histochemistry and allows unambiguous in vivo detection of mitophagy and mitochondrial…

• P
  Pfizer
• A
  AstraZeneca
• G
  GlaxoSmithKline
• W
  Wellcome

  Award: 101022/Z/13/Z
• MK
  Merck KGaA
• WT
  Wellcome Trust

  Award: 101022/Z/13/Z
• MR
  Medical Research Council

  Awards: MC_UU_12016, MC_UU_12016/4