Accurate design of megadalton-scale two-component icosahedral protein complexes
University of Washington · Howard Hughes Medical Institute · +3 more institutions
Abstract
Nature provides many examples of self- and co-assembling protein-based molecular machines, including icosahedral protein cages that serve as scaffolds, enzymes, and compartments for essential biochemical reactions and icosahedral virus capsids, which encapsidate and protect viral genomes and mediate entry into host cells. Inspired by these natural materials, we report the computational design and experimental characterization of co-assembling, two-component, 120-subunit icosahedral protein nanostructures with molecular weights (1.8 to 2.8 megadaltons) and dimensions (24 to 40 nanometers in diameter) comparable to those of small viral capsids. Electron microscopy, small-angle x-ray scattering, and x-ray…
Citation impact
- FWCI
- 60.59
- Percentile
- 100%
- References
- 63
Authors
11Topics & keywords
- Icosahedral symmetry
- Capsid
- Nanostructure
- Nanotechnology
- Characterization (materials science)
- Crystallography
- Protein subunit
- Component (thermodynamics)
Funding
- NSNational Science FoundationAwards: DE-FC02-02ER63421, P41 GM103403, CHE-1332907, 1332907, DE-AC02-05CH11231, DE-AC02-06CH11357, 0718124, DGE-0718124
- HHHoward Hughes Medical Institute
- UDU.S. Department of EnergyAwards: -AC02-05CH11231, AC02-06CH11357, 05CH11231, P41 GM103403, AC02-05CH11231, DE-AC02, 06CH11357, DE-AC02-05CH11231, DE-FC02-02ER63421, DE-AC02-06CH11357, FC02-02ER63421, DE-AC02-
- BABill and Melinda Gates Foundation
- TPTakeda Pharmaceutical Company
- NINational Institutes of HealthAwards: DE-FC02-02ER63421, P41 GM103403, GM103403, 5P41RR015301-10, DE-AC02-06CH11357, DE-AC02-05CH11231, 8 P41 GM103403-10
- DADefense Advanced Research Projects AgencyAward: W911NF-14-1-0162
- ARAdvanced Research Projects Agency
- NINational Institute of General Medical SciencesAwards: DE-AC02-05CH11231, DE-AC02-06CH11357, GM103403, P41 GM103403, P41 GM103403-10, 8 P41 GM103403-10
- NCNational Center for Research ResourcesAwards: 5P41RR015301-10, GM103403, DE-AC02-05CH11231
- DODivision of ChemistryAwards: DE-AC02-05CH11231, CHE-1332907
- AFAir Force Office of Scientific ResearchAward: FA950-12-10112
- LBLawrence Berkeley National LaboratoryAwards: DE-AC02-05CH11231, DE-AC02-06CH11357, 05CH11231, AC02-05CH11231