YTHDF2 destabilizes m6A-containing RNA through direct recruitment of the CCR4–NOT deadenylase complex
Chinese Academy of Sciences · Shanghai Institute of Nutrition and Health · +9 more institutions
Abstract
Methylation at the N6 position of adenosine (m(6)A) is the most abundant RNA modification within protein-coding and long noncoding RNAs in eukaryotes and is a reversible process with important biological functions. YT521-B homology domain family (YTHDF) proteins are the readers of m(6)A, the binding of which results in the alteration of the translation efficiency and stability of m(6)A-containing RNAs. However, the mechanism by which YTHDF proteins cause the degradation of m(6)A-containing RNAs is poorly understood. Here we report that m(6)A-containing RNAs exhibit accelerated deadenylation that is mediated by the CCR4-NOT deadenylase complex. We further show that YTHDF2 recruits the CCR4-NOT complex through a…
Citation impact
- FWCI
- 34.82
- Percentile
- 100%
- References
- 61
Authors
8- HDHao DuCorresponding
Chinese Academy of Sciences, Shanghai Institute of Nutrition and Health, Shanghai CASB Biotechnology (China), Shanghai Institutes for Biological Sciences, Shanghai Advanced Research Institute, Center for Excellence in Molecular Cell Science, Shanghai Institute of Applied Physics, University of Chinese Academy of Sciences
- YZYa Zhao
Chinese Academy of Sciences, Shanghai Institute of Nutrition and Health, Shanghai CASB Biotechnology (China), Shanghai Institutes for Biological Sciences, Shanghai Advanced Research Institute, Center for Excellence in Molecular Cell Science, Shanghai Institute of Applied Physics, University of Chinese Academy of Sciences
- JHJinqiu He
Fudan University
- YZYao Zhang
Shanghai Institute of Planned Parenthood Research
- HXHairui Xi
Shanghai University, Chinese Academy of Sciences, Shanghai Institute of Nutrition and Health, Shanghai CASB Biotechnology (China), Shanghai Institutes for Biological Sciences, Shanghai Advanced Research Institute, Center for Excellence in Molecular Cell Science, Shanghai Institute of Applied Physics, University of Chinese Academy of Sciences
Topics & keywords
- RNA
- Cell biology
- RNA-binding protein
- Long non-coding RNA
- Small nucleolar RNA
- Biology
- Translation (biology)
- Protein subunit