Microglia contact induces synapse formation in developing somatosensory cortex
Japan Agency for Medical Research and Development · National Institute for Physiological Sciences · +4 more institutions
Abstract
Microglia are the immune cells of the central nervous system that play important roles in brain pathologies. Microglia also help shape neuronal circuits during development, via phagocytosing weak synapses and regulating neurogenesis. Using in vivo multiphoton imaging of layer 2/3 pyramidal neurons in the developing somatosensory cortex, we demonstrate here that microglial contact with dendrites directly induces filopodia formation. This filopodia formation occurs only around postnatal day 8-10, a period of intense synaptogenesis and when microglia have an activated phenotype. Filopodia formation is preceded by contact-induced Ca(2+) transients and actin accumulation. Inhibition of microglia by genetic ablation…
Citation impact
- FWCI
- 28.17
- Percentile
- 100%
- References
- 58
Authors
10- AMAkiko MiyamotoCorresponding
Japan Agency for Medical Research and Development, National Institute for Physiological Sciences
- HWHiroaki Wake
The Graduate University for Advanced Studies, SOKENDAI, Japan Agency for Medical Research and Development, Japan Science and Technology Agency, National Institute for Physiological Sciences
- AIAyako Ishikawa
The Graduate University for Advanced Studies, SOKENDAI, National Institute for Physiological Sciences
- KEKei Eto
The Graduate University for Advanced Studies, SOKENDAI, Japan Agency for Medical Research and Development, National Institute for Physiological Sciences
- KSKeisuke Shibata
Japan Agency for Medical Research and Development, University of Yamanashi
Topics & keywords
- Microglia
- Filopodia
- Neurogenesis
- Neuroscience
- Synaptogenesis
- Somatosensory system
- Biology
- Biological neural network