Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
MRC Laboratory for Molecular Cell Biology · University College London · +1 more institution
Abstract
Despite significant progress, high-speed live-cell super-resolution studies remain limited to specialized optical setups, generally requiring intense phototoxic illumination. Here, we describe a new analytical approach, super-resolution radial fluctuations (SRRF), provided as a fast graphics processing unit-enabled ImageJ plugin. In the most challenging data sets for super-resolution, such as those obtained in low-illumination live-cell imaging with GFP, we show that SRRF is generally capable of achieving resolutions better than 150 nm. Meanwhile, for data sets similar to those obtained in PALM or STORM imaging, SRRF achieves resolutions approaching those of standard single-molecule localization analysis. The…
Citation impact
- FWCI
- 80.11
- Percentile
- 100%
- References
- 29
Authors
6- NGNils GustafssonCorresponding
MRC Laboratory for Molecular Cell Biology, University College London
- SJS J Culley
MRC Laboratory for Molecular Cell Biology, University College London
- GWGeorge W. Ashdown
King's College London
- DMDylan M. Owen
King's College London
- PMPedro M. Pereira
MRC Laboratory for Molecular Cell Biology, University College London
Topics & keywords
- Fluorophore
- STED microscopy
- Optics
- Resolution (logic)
- Microscopy
- Confocal
- Superresolution
- Image resolution