Distinct stages in stress granule assembly and disassembly

Stress granules are non-membrane bound RNA-protein (RNP) assemblies that form when translation initiation is limited and contain a biphasic structure with stable core structures surrounded by a less concentrated shell. The order of assembly and disassembly of these two structures remains unknown. Time course analysis of granule assembly suggests that core formation is an early event in granule assembly. Stress granule disassembly is also a stepwise process with shell dissipation followed by core clearance. Perturbations that alter liquid-liquid phase separations (LLPS) driven by intrinsically disordered protein regions (IDR) of RNA binding proteins in vitro have the opposite effect on stress granule assembly…

• HH
  Howard Hughes Medical Institute
• NI
  National Institute of General Medical Sciences

  Award: GM045443
• NI
  National Institute of Neurological Disorders and Stroke

  Award: F30N2093682