Oxford Nanopore MinION Sequencing and Genome Assembly
Chinese Academy of Sciences · National Center for Gene Research · +1 more institution
Abstract
The revolution of genome sequencing is continuing after the successful second-generation sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by Pacific Biosciences (PacBio), is progressing rapidly, moving from a technology once only capable of providing data for small genome analysis, or for performing targeted screening, to one that promises high quality de novo assembly and structural variation detection for human-sized genomes. In 2014, the MinION, the first commercial sequencer using nanopore technology, was released by Oxford Nanopore Technologies (ONT). MinION identifies DNA bases by measuring the changes in electrical conductivity generated as DNA strands pass through a…
Citation impact
- FWCI
- 41.60
- Percentile
- 100%
- References
- 65
Authors
3Topics & keywords
- Minion
- Nanopore sequencing
- Hybrid genome assembly
- Nanopore
- Sequence assembly
- Software portability
- Genome
- DNA sequencing