articleScientific ReportsSep 19, 2016GOLD OA

Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents

AGAna Gámez‐ValeroMTMarta TortajadaLCLaura Carreras‐PlanellaMFM. FranquesaKBKatrin Beyer

Universitat Autònoma de Barcelona · Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol · +1 more institution

PubMed
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Abstract

Extracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation has been the most widely used methodology but rapid methods, including Size Exclusion Chromatography (SEC) and/or precipitating agents such as Polyethylene glycol (PEG) or PRotein Organic Solvent PRecipitation (PROSPR) have emerged. To evaluate the impact of these different methods on the resulting EV preparations, plasma EVs were isolated using SEC, PEG and PROSPR, and their total protein content, NTA and Cryo-electron microscopy profiles, and EV-markers were compared. Also, their effect on recipient cells was tested. Low…

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Topics & keywords

Topics
Keywords
  • Extracellular vesicles
  • Isolation (microbiology)
  • Extracellular
  • Size-exclusion chromatography
  • Vesicle
  • Chemistry
  • Chromatography
  • Biology
UN Sustainable Development Goals
  • Reduced inequalities
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