Bone Formation by Sheep Stem Cells in an Ectopic Mouse Model: Comparison of Adipose and Bone Marrow Derived Cells and Identification of Donor‐Derived Bone by Antibody Staining

Background . Scaffolds for bone tissue engineering (BTE) can be loaded with stem and progenitor cells (SPC) from different sources to improve osteogenesis. SPC can be found in bone marrow, adipose tissue, and other tissues. Little is known about osteogenic potential of adipose‐derived culture expanded, adherent cells (A‐CEAC). This study compares in vivo osteogenic capacity between A‐CEAC and bone marrow derived culture expanded, adherent cells (BM‐CEAC). Method . A‐CEAC and BM‐CEAC were isolated from five female sheep and seeded on hydroxyapatite granules prior to subcutaneous implantation in immunodeficient mice. The doses of cells in the implants were 0.5 × 10 6 , 1.0 × 10 6 , or 1.5 × 10 6 A‐CEAC and 0.5 ×…

• SO
  Sundhed og Sygdom, Det Frie Forskningsråd

  Award: DFF– 4004-00256, MD
• DF
  Danmarks Frie Forskningsfond