High‐resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources
University of Massachusetts Chan Medical School · Massachusetts General Hospital · +2 more institutions
Abstract
Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for use in diagnostics, therapeutics and drug delivery. However, little is known about the relationship of protein and lipid composition of EVs and their source cells. Here, we report high-resolution lipidomic and proteomic analyses of exosomes and MVs derived by differential ultracentrifugation from 3 different cell types: U87 glioblastoma cells, Huh7 hepatocellular carcinoma cells and human bone marrow-derived mesenchymal stem cells (MSCs). We identified 3,532 proteins and 1,961 lipid species in the screen. Exosomes differed from MVs in several different areas: (a) The protein patterns of exosomes were more likely…
Citation impact
- FWCI
- 27.50
- Percentile
- 100%
- References
- 56
Authors
12- RAReka A. Haraszti
University of Massachusetts Chan Medical School
- MDMarie-Cécile Didiot
University of Massachusetts Chan Medical School
- ESEllen Sapp
Massachusetts General Hospital, MaineGeneral Medical Center
- JLJohn Leszyk
University of Massachusetts Chan Medical School
- SAScott A. Shaffer
University of Massachusetts Chan Medical School
Topics & keywords
- Microvesicles
- Microvesicle
- Extracellular vesicles
- Computational biology
- Biology
- Cell biology
- microRNA
- Genetics