Lipid Nanoparticle Assisted mRNA Delivery for Potent Cancer Immunotherapy

The induction of a strong cytotoxic T cell response is an important prerequisite for successful immunotherapy against many viral diseases and tumors. Nucleotide vaccines, including mRNA vaccines with their intracellular antigen synthesis, have been shown to be potent activators of a cytotoxic immune response. The intracellular delivery of mRNA vaccines to the cytosol of antigen presenting immune cells is still not sufficiently well understood. Here, we report on the development of a lipid nanoparticle formulation for the delivery of mRNA vaccines to induce a cytotoxic CD 8 T cell response. We show transfection of dendritic cells, macrophages, and neutrophils. The efficacy of the vaccine was tested in an…

• BW
  Burroughs Wellcome Fund

  Award: 1015145
• RI
  Ragon Institute of MGH, MIT and Harvard
• M
  Moderna
• M
  Max-Planck-Gesellschaft
• NI
  National Institutes of Health
• NC
  National Cancer Institute

  Award: F32CA200351