CTCF and cohesin regulate chromatin loop stability with distinct dynamics
Howard Hughes Medical Institute · University of California, Berkeley
Abstract
Folding of mammalian genomes into spatial domains is critical for gene regulation. The insulator protein CTCF and cohesin control domain location by folding domains into loop structures, which are widely thought to be stable. Combining genomic and biochemical approaches we show that CTCF and cohesin co-occupy the same sites and physically interact as a biochemically stable complex. However, using single-molecule imaging we find that CTCF binds chromatin much more dynamically than cohesin (~1-2 min vs. ~22 min residence time). Moreover, after unbinding, CTCF quickly rebinds another cognate site unlike cohesin for which the search process is long (~1 min vs. ~33 min). Thus, CTCF and cohesin form a rapidly…
Citation impact
- FWCI
- 27.70
- Percentile
- 100%
- References
- 85
Authors
5- ASAnders S. Hansen
Howard Hughes Medical Institute, University of California, Berkeley
- IPIryna Pustova
Howard Hughes Medical Institute, University of California, Berkeley
- CCClaudia Cattoglio
Howard Hughes Medical Institute, University of California, Berkeley
- RTRobert Tjian
Howard Hughes Medical Institute, University of California, Berkeley
- XDXavier DarzacqCorresponding
University of California, Berkeley
Topics & keywords
- Cohesin
- CTCF
- Chromatin
- Cell biology
- Biology
- Regulator
- Genetics
- Computational biology