DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins

The combined availability of whole genome sequences and genome editing tools is set to revolutionise the field of fruit biotechnology by enabling the introduction of targeted genetic changes with unprecedented control and accuracy, both to explore emergent phenotypes and to introduce new functionalities. Although plasmid-mediated delivery of genome editing components to plant cells is very efficient, it also presents some drawbacks, such as possible random integration of plasmid sequences in the host genome. Additionally, it may well be intercepted by current process-based GMO regulations, complicating the path to commercialisation of improved varieties. Here, we explore direct delivery of purified CRISPR/Cas9…

• FE
  Fondazione Edmund Mach
• PA
  Provincia Autonoma di Trento