A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors

Mammals have extremely limited regenerative capabilities; however, axolotls are profoundly regenerative and can replace entire limbs. The mechanisms underlying limb regeneration remain poorly understood, partly because the enormous and incompletely sequenced genomes of axolotls have hindered the study of genes facilitating regeneration. We assembled and annotated a de novo transcriptome using RNA-sequencing profiles for a broad spectrum of tissues that is estimated to have near-complete sequence information for 88% of axolotl genes. We devised expression analyses that identified the axolotl orthologs of cirbp and kazald1 as highly expressed and enriched in blastemas. Using morpholino anti-sense…

• HH
  Howard Hughes Medical Institute
• RA
  Richard and Susan Smith Family Foundation
• BA
  Brigham and Women's Hospital
• NI
  National Institutes of Health

  Awards: 1U24CA180922-01, P40-OD019794
• HS
  Harvard Stem Cell Institute
• NC
  National Cancer Institute