Next Generation Flow for highly sensitive and standardized detection of minimal residual disease in multiple myeloma
Universidad de Salamanca · Cancer Research Center · +18 more institutions
Abstract
Flow cytometry has become a highly valuable method to monitor minimal residual disease (MRD) and evaluate the depth of complete response (CR) in bone marrow (BM) of multiple myeloma (MM) after therapy. However, current flow-MRD has lower sensitivity than molecular methods and lacks standardization. Here we report on a novel next generation flow (NGF) approach for highly sensitive and standardized MRD detection in MM. An optimized 2-tube 8-color antibody panel was constructed in five cycles of design-evaluation-redesign. In addition, a bulk-lysis procedure was established for acquisition of ⩾107 cells/sample, and novel software tools were constructed for automatic plasma cell gating. Multicenter evaluation of…
Citation impact
- FWCI
- 41.77
- Percentile
- 100%
- References
- 58
Authors
33- JFJuan Flores‐MonteroCorresponding
Universidad de Salamanca, Cancer Research Center, Centro de Investigación del Cáncer
- LSLuzalba Sanoja-Flores
Universidad de Salamanca, Cancer Research Center, Centro de Investigación del Cáncer
- BPBruno Paiva
Clinica Universidad de Navarra, Universidad de Navarra
- NPNoemí Puig
Instituto de Investigación Biomédica de Salamanca, Centro de Investigación del Cáncer
- OGOmar García-Sánchez
Instituto de Investigación Biomédica de Salamanca, Centro de Investigación del Cáncer
Topics & keywords
- Minimal residual disease
- Medicine
- Multiple myeloma
- Flow cytometry
- Internal medicine
- Oncology
- Bone marrow
- Nuclear medicine