A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents

Exosomes play a role in cell-to-cell signaling and serve as possible biomarkers. Isolating exosomes with reliable quality and substantial concentration is a major challenge. Our purpose is to compare the exosomes extracted by three different exosome isolation kits (miRCURY, ExoQuick, and Invitrogen Total Exosome Isolation Reagent) and differential ultracentrifugation (UC) using six different volumes of a non-cancerous human serum (5 ml, 1 ml, 500 μl, 250 μl, 100 μl, and 50 μl) and three different volumes (1 ml, 500 μl and 100 μl) of six individual commercial serum samples collected from human donors. The smaller starting volumes (100 μl and 50 μl) are used to mimic conditions of limited availability of…

• GR
  Glaucoma Research Foundation

  Award: R01 EY023242
• GF
  Glaucoma Foundation
• BF
  BrightFocus Foundation
• AU
  Augusta University
• NI
  National Institutes of Health

  Awards: AG036675, P01 AG036675, EY023468, EY023242, NIH R01
• NI
  National Institute on Aging

  Awards: F32 AG044954, P01 AG036675
• NE
  National Eye Institute

  Awards: R01 EY023242, F32 EY023468