Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis
University of California, San Francisco · European Molecular Biology Laboratory · +8 more institutions
Abstract
A systems-level understanding of Gram-positive bacteria is important from both an environmental and health perspective and is most easily obtained when high-quality, validated genomic resources are available. To this end, we constructed two ordered, barcoded, erythromycin-resistance- and kanamycin-resistance-marked single-gene deletion libraries of the Gram-positive model organism, Bacillus subtilis. The libraries comprise 3,968 and 3,970 genes, respectively, and overlap in all but four genes. Using these libraries, we update the set of essential genes known for this organism, provide a comprehensive compendium of B. subtilis auxotrophic genes, and identify genes required for utilizing specific carbon and…
Citation impact
- FWCI
- 40.00
- Percentile
- 100%
- References
- 81
Authors
15Topics & keywords
- Bacillus subtilis
- Genome
- Biology
- Computational biology
- Genetics
- Scale (ratio)
- Gene
- Bacteria