Design of a synthetic yeast genome
Johns Hopkins University · High Throughput Biology (United States) · +3 more institutions
Abstract
Genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome…
Citation impact
- FWCI
- 31.51
- Percentile
- 100%
- References
- 44
Authors
12- SMSarah M. RichardsonCorresponding
Johns Hopkins University, High Throughput Biology (United States)
- LALeslie A. MitchellCorresponding
Johns Hopkins University, NYU Langone Health, High Throughput Biology (United States)
- GSGiovanni StracquadanioCorresponding
University of Essex, Johns Hopkins University, High Throughput Biology (United States)
- KYKun YangCorresponding
Johns Hopkins University, High Throughput Biology (United States)
- JSJessica S. Dymond
Johns Hopkins University, High Throughput Biology (United States)
Topics & keywords
- Genome
- Synthetic biology
- Chromosome
- Computational biology
- Saccharomyces cerevisiae
- Biology
- Genetics
- Genome evolution