Engineering canker‐resistant plants through CRISPR /Cas9‐targeted editing of the susceptibility gene Cs LOB 1 promoter in citrus
Southwest University · Citrus Research Institute · +1 more institution
Abstract
Summary Citrus canker, caused by Xanthomonas citri subsp. citri ( Xcc ), is severely damaging to the global citrus industry. Targeted editing of host disease‐susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistance through CRISPR /Cas9‐targeted modification of the susceptibility gene Cs LOB 1 promoter in citrus. Wanjincheng orange ( Citrus sinensis Osbeck) harbours at least three copies of the Cs LOB 1 G allele and one copy of the Cs LOB 1 − allele. The promoter of both alleles contains the effector binding element ( EBE P thA4 ), which is recognized by the main effector PthA4 of Xcc to activate…
Citation impact
- FWCI
- 22.99
- Percentile
- 100%
- References
- 50
Authors
8- APAihong Peng
Southwest University, Citrus Research Institute, Chinese Academy of Agricultural Sciences
- SCShanchun Chen
Southwest University, Citrus Research Institute, Chinese Academy of Agricultural Sciences
- TLTiangang Lei
Southwest University, Citrus Research Institute, Chinese Academy of Agricultural Sciences
- LXLanzhen Xu
Southwest University, Citrus Research Institute, Chinese Academy of Agricultural Sciences
- YHYongrui He
Southwest University, Citrus Research Institute, Chinese Academy of Agricultural Sciences
Topics & keywords
- Citrus canker
- Biology
- Xanthomonas citri
- Canker
- Effector
- Gene
- Mutant
- Citrus × sinensis
- Industry, innovation and infrastructure