articleScienceMar 30, 2017GREEN OA

Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq

ASAndrew S. VenteicherITItay TiroshCHChristine HebertKYKeren YizhakCNCyril Neftel

Broad Institute · Harvard University · +8 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Tumor subclasses differ according to the genotypes and phenotypes of malignant cells as well as the composition of the tumor microenvironment (TME). We dissected these influences in isocitrate dehydrogenase (IDH)-mutant gliomas by combining 14,226 single-cell RNA sequencing (RNA-seq) profiles from 16 patient samples with bulk RNA-seq profiles from 165 patient samples. Differences in bulk profiles between IDH-mutant astrocytoma and oligodendroglioma can be primarily explained by distinct TME and signature genetic events, whereas both tumor types share similar developmental hierarchies and lineages of glial differentiation. As tumor grade increases, we find enhanced proliferation of malignant cells, larger pools…

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1,052
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39.13
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100%
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Authors

34

Topics & keywords

Topics
Keywords
  • Isocitrate dehydrogenase
  • Biology
  • Oligodendroglioma
  • IDH1
  • Glioma
  • Mutant
  • RNA
  • Phenotype
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