Quantitative Multiplex Immunohistochemistry Reveals Myeloid-Inflamed Tumor-Immune Complexity Associated with Poor Prognosis

Here, we describe a multiplexed immunohistochemical platform with computational image processing workflows, including image cytometry, enabling simultaneous evaluation of 12 biomarkers in one formalin-fixed paraffin-embedded tissue section. To validate this platform, we used tissue microarrays containing 38 archival head and neck squamous cell carcinomas and revealed differential immune profiles based on lymphoid and myeloid cell densities, correlating with human papilloma virus status and prognosis. Based on these results, we investigated 24 pancreatic ductal adenocarcinomas from patients who received neoadjuvant GVAX vaccination and revealed that response to therapy correlated with degree of mono-myelocytic…

• UD
  U.S. Department of Defense
• BC
  Breast Cancer Research Foundation
• LF
  Lustgarten Foundation
• OH
  Oregon Health and Science University

  Award: P30 CA069533-17
• NI
  National Institutes of Health
• OC
  Oregon Clinical and Translational Research Institute

  Award: UL1TR000128
• NC
  National Cancer Institute

  Award: CA192405
• NC
  National Center for Advancing Translational Sciences