Nucleic acid detection with CRISPR-Cas13a/C2c2

Gootenberg, Jonathan S.; Abudayyeh, Omar O.; Lee, Jeong Wook; Essletzbichler, Patrick; Dy, Aaron J.; Joung, Julia; Verdine, Vanessa K.; Donghia, Nina M.; Daringer, Nichole M.; Freije, Catherine A.; Myhrvold, Cameron; Bhattacharyya, Roby P.; Livny, Jonathan; Regev, Aviv; Koonin, Eugene V.; Hung, Deborah T.; Sabeti, Pardis C.; Collins, James J.; Zhang, Feng

doi:10.1126/science.aam9321

articleScienceApr 13, 2017GREEN OA

Nucleic acid detection with CRISPR-Cas13a/C2c2

JSJonathan S. Gootenberg OOOmar O. Abudayyeh JWJeong Wook Lee PEPatrick Essletzbichler AJAaron J. Dy

Broad Institute · Harvard University · +6 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral effect of Cas13a with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity. We use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing…

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3,767

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FWCI: 81.18
Percentile: 100%
References: 36

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Authors

19

Topics & keywords

Topics

Keywords

CRISPR
Recombinase Polymerase Amplification
Nucleic acid
Genotyping
DNA
Biology
Computational biology
Polymerase chain reaction

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