A chromosome conformation capture ordered sequence of the barley genome

Cereal grasses of the Triticeae tribe have been the major food source in temperate regions since the dawn of agriculture. Their large genomes are characterized by a high content of repetitive elements and large pericentromeric regions that are virtually devoid of meiotic recombination. Here we present a high-quality reference genome assembly for barley (Hordeum vulgare L.). We use chromosome conformation capture mapping to derive the linear order of sequences across the pericentromeric space and to investigate the spatial organization of chromatin in the nucleus at megabase resolution. The composition of genes and repetitive elements differs between distal and proximal regions. Gene family analyses reveal…

• NS
  National Science Foundation

  Awards: 0315954, 1062301, 0321756
• UD
  U.S. Department of Agriculture

  Award: 2011-68002-30029
• GR
  Grains Research and Development Corporation

  Award: DAW00233
• NN
  National Natural Science Foundation of China

  Awards: 31330055, 31129005
• GO
  Government of Western Australia
• DF
  Directorate for Biological Sciences
• NI
  National Institute of Food and Agriculture

  Award: 2011-68002-30029
• CS
  Cooperative State Research, Education, and Extension Service
• BA
  Biotechnology and Biological Sciences Research Council

  Awards: BB/I00663X/1, BB/I008357/1, BB/I008071/1, BB/J004669/1, BB/I008071/1, BB/J010375/1, BBS/E/T/000PR5885, BBS/E/T/000PR6193, BB/H531519/1, BB/L026317/1, BB/I008357/1
• L
  Leibniz-Gemeinschaft