Single-cell RNA-seq enables comprehensive tumour and immune cell profiling in primary breast cancer
Samsung Medical Center · Sungkyunkwan University · +3 more institutions
Abstract
Single-cell transcriptome profiling of tumour tissue isolates allows the characterization of heterogeneous tumour cells along with neighbouring stromal and immune cells. Here we adopt this powerful approach to breast cancer and analyse 515 cells from 11 patients. Inferred copy number variations from the single-cell RNA-seq data separate carcinoma cells from non-cancer cells. At a single-cell resolution, carcinoma cells display common signatures within the tumour as well as intratumoral heterogeneity regarding breast cancer subtype and crucial cancer-related pathways. Most of the non-cancer cells are immune cells, with three distinct clusters of T lymphocytes, B lymphocytes and macrophages. T lymphocytes and…
Citation impact
- FWCI
- 34.31
- Percentile
- 100%
- References
- 62
Authors
13- WCWoosung ChungCorresponding
Samsung Medical Center, Sungkyunkwan University
- HHHye Hyeon Eum
Seoul National University, Samsung Medical Center
- HLHae‐Ock Lee
Seoul National University, Samsung Medical Center, Sungkyunkwan University
- KMKyung Min Lee
Seoul National University, Seoul National University Hospital
- HLHan‐Byoel Lee
Seoul National University, Samsung Medical Center, Sungkyunkwan University
Topics & keywords
- Immune system
- Stromal cell
- Transcriptome
- Biology
- Cancer cell
- Phenotype
- Breast cancer
- Cancer research
- Good health and well-being