articleScienceMay 11, 2017Closed access

A subcellular map of the human proteome

PTPeter ThulLÅLovisa ÅkessonMWMikaela WikingDMDiana MahdessianAGAikaterini Geladaki

Science for Life Laboratory · KTH Royal Institute of Technology · +4 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments.…

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Topics & keywords

Topics
Keywords
  • Proteome
  • Human proteome project
  • Human Protein Atlas
  • Computational biology
  • Proteomics
  • Context (archaeology)
  • Human proteins
  • Function (biology)
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