YAP regulates cell mechanics by controlling focal adhesion assembly

Hippo effectors YAP/TAZ act as on-off mechanosensing switches by sensing modifications in extracellular matrix (ECM) composition and mechanics. The regulation of their activity has been described by a hierarchical model in which elements of Hippo pathway are under the control of focal adhesions (FAs). Here we unveil the molecular mechanism by which cell spreading and RhoA GTPase activity control FA formation through YAP to stabilize the anchorage of the actin cytoskeleton to the cell membrane. This mechanism requires YAP co-transcriptional function and involves the activation of genes encoding for integrins and FA docking proteins. Tuning YAP transcriptional activity leads to the modification of cell…

• GF
  Graphene Flagship

  Award: 696656
• EC
  European Commission

  Awards: 696656, 732344, CZ.02.1.01, H2020, 693670, CZ.1.05/1.1.00/02.0068
• MŠ
  Ministerstvo Školství, Mládeže a Tělovýchovy

  Awards: CZ.1.05/1.1.00/02.0068, LQ1605, LQ1601, CZ.02.1.01/0.0/0.0/15_003, CZ.02.1.01, CZ.1.05, CZ.1.05/1.1.00/02.0123, LO1304
• CE
  Central European Institute of Technology

  Awards: LQ1601, CZ.1.05/1.1.00/02.0068, CZ.1.05/1.1.00/02
• H2
  Horizon 2020 Framework Programme

  Awards: 732344, No. 696656, LQ1601, 696656, Graphene Flagship Core 1 No. 696656
• ER
  European Regional Development Fund

  Awards: 02.1.01/0.0/0.0, H2020, CZ.02, CZ.02.1.01, CZ.02.1.01/0.0/0.0, CZ.1.05/1.1.00/02.0123, CZ.1.05/1.1.00/02.0068, LQ1601, 1.05/1.1.00/02.0068, CZ.02.1.01/0.0/0.0/15_003/0000492
• ES
  European Social Fund

  Awards: CZ.1.05/1.1.00/02.0068, LO1304, CZ.02.1.01