Metabolic network analysis reveals microbial community interactions in anammox granules

Abstract Microbial communities mediating anaerobic ammonium oxidation (anammox) represent one of the most energy-efficient environmental biotechnologies for nitrogen removal from wastewater. However, little is known about the functional role heterotrophic bacteria play in anammox granules. Here, we use genome-centric metagenomics to recover 17 draft genomes of anammox and heterotrophic bacteria from a laboratory-scale anammox bioreactor. We combine metabolic network reconstruction with metatranscriptomics to examine the gene expression of anammox and heterotrophic bacteria and to identify their potential interactions. We find that Chlorobi-affiliated bacteria may be highly active protein degraders,…

• NS
  National Science Foundation

  Awards: 1435661, MCB-1518130, 1518130
• NS
  Natural Sciences and Engineering Research Council of Canada
• DO
  Division of Chemical, Bioengineering, Environmental, and Transport Systems

  Award: CBET-1435661