Oligolysine-based coating protects DNA nanostructures from low-salt denaturation and nuclease degradation
Harvard University · Dana-Farber Cancer Institute · +4 more institutions
Abstract
DNA nanostructures have evoked great interest as potential therapeutics and diagnostics due to ease and robustness of programming their shapes, site-specific functionalizations and responsive behaviours. However, their utility in biological fluids can be compromised through denaturation induced by physiological salt concentrations and degradation mediated by nucleases. Here we demonstrate that DNA nanostructures coated by oligolysines to 0.5:1 N:P (ratio of nitrogen in lysine to phosphorus in DNA), are stable in low salt and up to tenfold more resistant to DNase I digestion than when uncoated. Higher N:P ratios can lead to aggregation, but this can be circumvented by coating instead with an oligolysine-PEG…
Citation impact
- FWCI
- 19.85
- Percentile
- 100%
- References
- 33
Authors
10- NPNandhini PonnuswamyCorresponding
Harvard University, Dana-Farber Cancer Institute, Inspire Institute
- MMMaartje M. C. Bastings
Harvard University, Dana-Farber Cancer Institute, Inspire Institute
- BNBhavik Nathwani
Harvard University, Dana-Farber Cancer Institute, Inspire Institute
- JHJu Hee Ryu
Harvard University, Dana-Farber Cancer Institute, Inspire Institute, Korea Institute of Science and Technology
- LYLeo Y. T. Chou
Harvard University, Dana-Farber Cancer Institute, Inspire Institute
Topics & keywords
- Nuclease
- Degradation (telecommunications)
- DNA
- Coating
- Denaturation (fissile materials)
- Nanostructure
- Chemistry
- Materials science