Protein–Sol: a web tool for predicting protein solubility from sequence

MOTIVATION: Protein solubility is an important property in industrial and therapeutic applications. Prediction is a challenge, despite a growing understanding of the relevant physicochemical properties. RESULTS: Protein-Sol is a web server for predicting protein solubility. Using available data for Escherichia coli protein solubility in a cell-free expression system, 35 sequence-based properties are calculated. Feature weights are determined from separation of low and high solubility subsets. The model returns a predicted solubility and an indication of the features which deviate most from average values. Two other properties are profiled in windowed calculation along the sequence: fold propensity, and net…

• DF
  Directorate for Biological Sciences
• EA
  Engineering and Physical Sciences Research Council

  Awards: BB/L013770/1, BB/J014478/1, EP/N024796/1
• BA
  Biotechnology and Biological Sciences Research Council

  Awards: BB/J014478/1, BB/L013770/1, BB/L013770/1, BB/I017194/1