m 6 A mRNA modifications are deposited in nascent pre-mRNA and are not required for splicing but do specify cytoplasmic turnover
Howard Hughes Medical Institute · Rockefeller University · +3 more institutions
Abstract
Understanding the biologic role of N 6 -methyladenosine (m 6 A) RNA modifications in mRNA requires an understanding of when and where in the life of a pre-mRNA transcript the modifications are made. We found that HeLa cell chromatin-associated nascent pre-mRNA (CA-RNA) contains many unspliced introns and m 6 A in exons but very rarely in introns. The m 6 A methylation is essentially completed upon the release of mRNA into the nucleoplasm. Furthermore, the content and location of each m 6 A modification in steady-state cytoplasmic mRNA are largely indistinguishable from those in the newly synthesized CA-RNA or nucleoplasmic mRNA. This result suggests that quantitatively little methylation or demethylation…
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Authors
10- SKShengdong KeCorresponding
Howard Hughes Medical Institute, Rockefeller University
- APAmy Pandya‐Jones
University of California, Los Angeles
- YSYuhki Saito
Howard Hughes Medical Institute, Rockefeller University
- JJJohn J. Fak
Howard Hughes Medical Institute, Rockefeller University
- CBCathrine Broberg Vågbø
Norwegian University of Science and Technology
Topics & keywords
- Biology
- RNA splicing
- Messenger RNA
- Precursor mRNA
- Exon
- Molecular biology
- Intron
- RNA